The church of Santa Maria Assunta is situated in the district of Poggio di Loro in Loro Ciuffenna, Tuscany. The building, of Roman origin even if it was partially rehashed, presents a sandstone gabled façade architecture typical of mountain buildings, which has a single nave with an apse and roof trusses. On the façade, the ancient masonry seems almost intact, on which there is a narrow architraved portal surmounted by a modern double-arched window. Inside the church, there is the polyptych with the Madonna and Child among the four Evangelists. The work, which should date back to the fourth decade of the 1400s, was firstly attributed by the critics to Andrea di Giusto, then to Mariotto di Cristofano and lastly to Stefano d’Antonio Vanni.